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KMID : 0357119930150010045
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Korean Journal of Immunology
1993 Volume.15 No. 1 p.45 ~ p.51
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Regulation of Mouse Macrophage Fc Receptor-mediated Phagocytosis by Interferon-γ, Lipid A and Tumor Necrosis Factor
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Kim Joong-Gon
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Abstract
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Relation of mouse macrophage Fc receptor-mediated phagocytosis by interferon-γ, lipid A and tumor necrosis factor was investigated. Pretreatment of macropahges with lipid A or IFN-γ increased the FcR-mediated phagocytosis of IgG2a-opsonized sheep erythrocyte conjugates.FcR-mediated phagocytosis was significantly reduced by γTNF at the high concentration (1,000U/ml), but slightly enhanced by the low concentratijon of γTNF (15U/ml). γTNF slightly reduced the increased FcRd-mediated phagocytosis induced by IFN-γ. Although both lipid A and IFN-γ had the up-regulatory effects on the FcR-mediated phagocytosis, culture of macrophages with IFN-γand lipid A decreased the phagocytic activity of macrophages below of that stimulated with IFN-γalone, but above that of control values. Since lipid A alone or in combination with IFN-γ induced the production of TNF by lmacrophages, the evffect of endogenous TNF on the RcR-mediated phagocytosis was observed by adding the rabbit anti-mouse IFN-γ antibody to the macrophages stimulated with IFN-γ and lipid A. Addition of anti-TNF antibody restored the phagocytosis downregulated by lipid A to that sftimulated with IFN-γ alone. This finding suggests that the production of high level of TNF in response to IFN-γand lipid A appears to down-regulate FcR-mediated phagocytosis.
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KEYWORD
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Macrophage, Fc Receptor, Phagocytosis
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